Fig. 4

MTX and DOX induce YAP nucleus translocation. a, b MG63 and U2OS cells cultured on coverslips were exposed to MTX (100 μM) or DOX (1.5 μM) for 24 h. Endogenous YAP was stained using an anti-YAP antibody (red), and nuclei were stained with DAPI (blue). The subcellular localization of YAP was quantified (lower panels). The error bars represent mean ± SD. Compared with control (N nucleus; C cytoplasm. t test, n = 100), **P < 0.01, ^## P < 0.01. Results show that MTX and DOX promoted YAP nuclear translocation. c, d Co-immunoprecipitation was applied to investigate the interaction between YAP and 14-3-3β in MG63 and U2OS cells. Cells were exposed to indicate concentrations of MTX or DOX for 24 h before co-immunoprecipitation. The results were determined by western blotting and the interaction between YAP and 14-3-3β was decreased by MTX and DOX