Fig. 2
From: Hippo/YAP signaling pathway is involved in osteosarcoma chemoresistance
MTX and DOX induce YAP activation in MG63 and U2OS osteosarcoma cells. a, b Left panel Western blotting representative image of large tumor suppressor kinase 2 (LATS2), YAP, and YAP phosphorylation at Ser127 in MG63 cells with indicated concentrations of MTX or DOX treatments. Right panel Grayscale comparison of phosphorylated YAP to YAP total protein. Data are shown as mean ± SD. Compared with control (t test, n = 3), **P < 0.01. YAP phosphorylation level at Ser127 and LATS2 protein level in MG63 cells was decreased by MTX and DOX. c, d Left panel Western blotting representative image of LATS2, YAP, and YAP phosphorylation at Ser127 in U2OS cells with indicated concentrations of MTX or DOX treatments. Right panel Grayscale comparison of phosphorylated YAP to YAP total protein. Data are shown as mean ± SD. Compared with control (t test, n = 3), **P < 0.01. YAP phosphorylation level at Ser127 and LATS2 protein level in U2OS cells was decreased by MTX and DOX. P-YAP YAP phosphorylation