Fig. 1

Yes-associated protein (YAP) increases the proliferation and chemoresistance of MG63 osteosarcoma cells. a Control and YAP-overexpressing/knockdown MG63 cells were seeded at the same concentration, and cell numbers were counted every 24 h. Data are shown as mean ± standard deviation (SD). Compared with control (t test, n = 3), **P < 0.01, and *P < 0.05. Results show that overexpression of YAP accelerated MG63 cell proliferation and knockdown of YAP decreased cell proliferation. b Cell viability was analyzed by detecting total cellular adenosine triphosphate in methotrexate (MTX) (20 mM)- or doxorubicin (DOX) (10 μM)-treated control and YAP-overexpressing MG63 cells. Data are shown as mean ± SD. Compared with control (t test, n = 3), **P < 0.01, and ^## P < 0.01. Overexpression of YAP increased the viability of MG63 cells treated with MTX (20 mM) or DOX (10 μM). c Left panel representative images of flow cytometry analysis of YAP-overexpressing and YAP-knockdown MG63 cells treated with MTX (20 mM) or DOX (10 μM) and stained by Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide. Due to the natural fluorescence of DOX, we calculated only Annexin V-FITC-positive cells in DOX-treated cells. Right panel Quantitative analysis of apoptosis percentages according to the results of left panel. Data are shown as mean ± SD. Compared with control (t test, n = 3), *P < 0.05, **P < 0.01, ^# P < 0.05, and ^## P < 0.01. The flow cytometry results showed that YAP increased the chemoresistance of osteosarcoma cells and knockdown of YAP increased the chemosensitivity of osteosarcoma cells. YAP-KD, knockdown of YAP