Table 3 Primers for polymerase chain reaction (PCR) amplification and subsequent P53 sequencing reactions
From: Why some tumours trigger neovascularisation and others don’t: the story thus far
Primer | Sequence | Annealing temperature (°C) | Amplicon length (bp) |
|---|---|---|---|
Exon 3–4 | Forward: GTGGGAAGCGAAAATTCCAT Reverse: GCCAGGCATTGAAGTCTCAT | 60 | 506 |
Exon 5–6 | Forward: TGTTCACTTGTGCCCTGACT Reverse: TTAACCCCTCCTCCCAGAGA | 60 | 467 |
Exon 7 | Forward: GAGCTTGCAGTGAGCTGAGA Reverse: GGGATGTGATGAGAGGTGGA EX7F_seq CCTGCTTGCCACAGGTCT (to be used for sequencing instead of Exon 7 forward primer) | 61.5 | 390 |
Exon 8–9 | Forward: GACAAGGGTGGTTGGGAGTA Reverse: GCCCCAATTGCAGGTAAAAC | 60 | 500 |