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Table 1 Antibodies used for immunohistochemistry

From: Why some tumours trigger neovascularisation and others don’t: the story thus far

Antigen Clone Source
HIF1 ESEE122 Abcam (Cambridge, UK)
HIF2 EP109b NDCLS (University of Oxford, John Radcliffe Hospital, Oxford, UK)
CA9 M75 BioScience Slovakia s.r.o. (Bratislava, Slovakia)
VEGFA VG-1 NDCLS
TYMP PGF44 NDCLS
KDR FLK1(A3) Santa Cruz Biotechnology, Inc. (Dallas, TX, USA)
KDRp34 34a NDCLS
FIH FIH162c/D6 NDCLS
PHD1 PHD112/G NDCLS
PHD2 366G/76 NDCLS
PHD3 EG188c NDCLS
DLL4 D4/37 NDCLS
TSP1 8A6B-TSP-1 Leica Microsystems (UK) Ltd. and Novocastra Reagents (Milton Keynes, UK)
CXCR4 MAB 172 R&D System (Minneapolis, MN, USA)
EPHB2 AF496 R&D System
EPHB3 R&D AF432 R&D System
EPHB4 R&D AF446 R&D System
SOD1 30F11 Novocastra
BCL2 124 Dako (Cambridgeshire, UK)
FOS Polyclonal 27436 Abcam
EGF EGF 10 Abcam
EGFR F4 Abcam
BNIP3 Ana40 Sigma-Aldrich Company Ltd. (Dorset, UK)
P53 DO7 Dako
PI3 Ab 40755 Abcam
SP1 SP1 polyclonal Abcam
STAT3 E121-21 Abcam
LON 20H1 NDCLS
MEF2D MEF2D polyclonal Abcam
JMY HMY117A NDCLS
TRAP1 TR-1A LabVision (TorsbySjöväg, Värmdö, Sweden)
GST GST3/GST pi Abcam
NCAM IB6 Novocastra
CHGA FLEX polyclonal Dako
SYP 299 Novocastra
  1. HIF hypoxia-inducible factor; CA9 carbonic anhydrase 9; VEGFA vascular endothelial growth factor A; TYMP thymidine phosphorylase; KDR vascular endothelial growth factor receptor 2; KDRp34 vascular endothelial growth factor p34; FIH factor-inhibiting HIF; PHD prolyl hydroxylase dehydrogenase; DLL4 Delta-like 4; TSP1 thymidine phosphorylase 1; CXCR4 chemokine (C-X-C motif) receptor 4; EPH ephrin; SOD1 superoxide dismutase 1; BCL2 B cell lymphoma 2; FOS FBJ murine osteosarcoma viral oncogene homolog; EGF epidermal growth factor; EGFR epidermal growth factor receptor; BNIP3 BCL2/adenovirus E1B 19 kDa interacting protein 3; PI3 peptidase inhibitor 3; SP1 Sp1 transcription factor; STAT3 signal transducer and activator of transcription 3; LON Lon protease; MEF2D myocyte enhancer factor 2D; JMY junction-mediating and regulatory protein, p53 cofactor; TRAP1 TNF receptor-associated protein 1; GST glutathione S-transferase; NCAM neural cell adhesion molecule; CHGA chromogranin A; SYP synaptophysin