Fig. 2

Stability of ES variants under certain conditions. a Trypsin digestion profiles of ES variants for the indicated time. b and c Proteins were incubated with different concentrations of guanidinium chloride (GdmCl), and GdmCl-induced denaturation of ES variants was monitored with tryptophan emission fluorescence intensity at 318 nm. b Raw data and fitted data of the unfolding process of ES, ZBP-ES (upper panel), and M₂ES, MZBP-ES (lower panel). c Normalized data of the GdmCl-induced unfolding of ES variants