Fig. 2

Effect of the RPMS1 SNP G155391A on the degradation of RPMS1 protein. a Western blotting analysis showing the expression levels of RPMS1 in NP69 cell lines established with the stable integration of the pBABE-Puro retroviral vector of mutant RPMS1 (-Mut), wild-type RPMS1 (-WT), and control vector (-Vec), respectively. b Western blotting results showing the degradation of RPMS1 protein. NP69 cells with stable overexpression of mutant RPMS1 (-Mut) or wild-type RPMS1 (-WT) were incubated with 20 μg/mL cycloheximide (CHX) for the indicated periods of time (0, 0.5, 1.0, 1.5, 2.0, and 2.5 h). c Fitted curves of the degradation of the RPMS1 protein of EBV variations under the damped exponential model. d Western blotting results showing the RPMS1 protein expression in NP69 cells with stable overexpression of mutant RPMS1 (-Mut) or wild-type RPMS1 (-WT), treated with or without 10 μmol/L MG132 for 1 h