Fig. 2From: Association of insulin-like growth factor-binding protein-3 with radiotherapy response and prognosis of esophageal squamous cell carcinomaImmunohistochemical analysis of IGFBP-3 staining and western blotting analysis. a Strong IGFBP-3 staining in an ESCC case (case 16), in which approximately 80% of ESCC cells are stained positively for IGFBP-3 protein in the cytoplasm (×200). b Weak IGFBP-3 staining in an ESCC case (case 10) (×200). c Negative IGFBP-3 staining in a non-malignant esophageal mucosa specimen (×200). d Strong IGFBP-3 staining in a non-malignant esophageal mucosa specimen (×200). e 7 of 10 ESCC cases displayed low expression of IGFBP-3 by western blotting compared with adjacent non-malignant esophageal tissues (N1–10)Back to article page